LIPO-STEM

Adipose tissue MSCs purification kit

 

LIPO-STEM is a closed-circuit single-use kit for adipose tissue purification without any centrifuge and with a minimal manipulation.

The entire processing phase of the liposuctioned tissue occurs inside the device thanks to continuous saline solution washing. This allows to reduce the cellular stress eliminating any traumatic action that may damage the extracellular matrix and its essential trophic and anti-inflammatory function.

The sophisticated filtering and washing system preserves the entire vascular stromal niche architecture and volume of the lipoaspirate and improves the cells’ capacity to respond to regenerative stimuli.

The final purified product is a viscous fluid that keeps the biological properties of the original tissue intact. The whole extracellular matrix acts as natural structure for the cells increasing their vitality and contributing to the natural tissue regeneration process.

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    KEY ADVANTAGES
     
    • No centrifugation required

    • Adipose tissue purification

    • Complete kit for sampling and processing adipose tissue and mesenchymal cells

    • Complete preservation of tissue architecture and stromal niche components

    • Reduced cellular stress

    • Preservation of the extracellular matrix 

    • Total purification of blood and oily waste as potential cause of inflammation

    • Minimal manipulation thanks to a simple and reproducible technique in a single surgical time

    FIELDS OF APPLICATION
     
    • ORTHOPAEDICS

    • MAXILLOFACIAL SURGERY

    • SPINAL SURGERY

    • PAIN THERAPY

    • VULNOTHERAPY

    • PLASTIC AND RECONSTRUCTIVE SURGERY

    • GENERAL/UROGYNAECOLOGICAL SURGERY

    SURGICAL TECHNIQUE

    1. Infiltrate the Klein solution in the selected harvest site using the 16 G Klein solution cannula.

    2. Harvest the adipose tissue from the anesthetized area using the 13 G lipoaspiration cannula.

    3. Connect a 2L saline bag to the device’s “WASH IN” valve and fill the device.

    4. Insert the harvested adipose tissue in the device’s “LIPO IN” valve.

    5. Support the purification of the adipose tissue using the spatula provided with the kit, with a gentle movement over the filter.

    6. Once the final product acquires a clear yellow color, stop the saline solution flow.

    7. Retrieve the purified adipose tissue from the dedicated “LIPO OUT” valve with a syringe and use as needed.

    COMPONENTS

    – LIPO-STEM device

    The device is supplied ready-to-use with a 400 ml capacity processing bag and a 2 lt waste collecting bag. The kit includes a spatula to facilitate the tissue purification.

    The system has 3 valves: 

           – WASH IN: to connect the saline solution

          – LIPO IN: to transfer the harvested adipose tissue into the device

          – LIPO OUT: to extract the purified adipose tissue.

    IN VALVE

    OUT VALVE

    – 16 G Klein solution cannula with 60 ml syringe

    The kit includes:

    • two 60 ml syringes for Klein solution infiltration
    • one 16 G cannula with atraumatic tip and three side holes for Klein solution infiltration.

    16 G CANNULA TIP

    – 13 G Atraumatic lipo-aspiration cannula with 60 ml Vaclok syringe

    The kit includes:

    • two 60 ml Vaclok syringes for lipoaspiration
    • one 13 G lipoaspiration cannula with atraumatic tip and three ultrasharp side holes that allow to harvest a high qualitative and vital adipose cluster.
    • 11 G lipo-aspiration cannula available upon request. 

    13 G or 11 G CANNULA TIP

    VIDEO TUTORIAL

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