Orthobiologics Procedures
MSCs COLLECTION
FROM ADIPOSE TISSUE
Mesenchymal stem cells from adipose tissue (ASCs) hold the capacity to differentiate into various cell types, including osteocytes, chondrocytes, and adipocytes, making them invaluable for mending damaged tissues. Moreover, they release growth factors that foster tissue regeneration. Beyond repair, ASCs display anti-inflammatory prowess, modulating immune cell activity.
This versatility renders autologous fat tissue implantation, a minimally invasive procedure that harnesses a patient’s fat tissue, a promising option for addressing degenerative conditions like osteoarthritis, tendon and ligament repair, and soft tissue reconstruction.
Enter LIPO-STEM DUO™, specialized, closed-circuit, disposable system designed for lipoaspirate collection, purification, and microfragmentation without the use of enzymes or centrifugation. This device preserves the structural integrity of vascular stromal niches within lipoaspirate while enhancing the cells’ responsiveness to regenerative signals.
Surgical procedure
1.
Infiltrate the Klein solution
Begin by infiltrating the Klein solution into the target area to facilitate fat harvesting and minimize discomfort or bleeding.
2.
Harvest the adipose tissue
Using a VacLok syringe, gently harvest the adipose tissue. Apply a soft, controlled negative pressure by locking the syringe in 10 cc increments.
3.
PREPARE THE WASHING SYSTEM
Close the waste line clamp on the bag tube. Then, connect a 2L saline bag to the device’s “WASH IN” valve and fill the system with approximately 150 ml of saline solution.
4.
INTRODUCE THE HARVESTED FAT
Insert the collected adipose tissue into the device via the “LIPO IN” valve. Open the waste line clamp to flush out impurities and excess fluids.
5.
WASH AND PURIFY THE FAT
Use the internal spatula to gently move the fat up and down. Continue until the fat turns pale yellow and the waste liquid appears transparent, indicating proper purification.
6.
RETRIEVE THE PURIFIED TISSUE
Close the infusion line clamp and perform one final spatula movement to collect remaining fat. Retrieve the purified adipose tissue through the “LIPO OUT” valve, ready for use.
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