Cryopreservation vitrification carrier

Vitrification is an advanced cryopreservation technique used in Assisted Reproduction Technology (ART) to preserve oocytes and embryos by rapidly cooling them to extremely low temperatures. Unlike traditional slow freezing, vitrification prevents ice crystal formation, which could damage the cells.

How to use:

COOLING

• Prepare a suitable container with clean (preferably Sterile Certified) liquid nitrogen (LN₂) and the selected vessel for holding the SUPERVITRI™ during cryostorage.
• Prepare the specimens for vitrification according to the recommendations of the selected media kit.
• Using a micropipette, carefully load a maximum of three (3) oocytes/embryos in a minimal volume (< 0.5 µl) onto the concave surface near the tip end of the device.
• When performing procedures in liquid nitrogen, always handle the SUPERVITRI™ device and its cap using sterile stainless-steel tweezers.

The SUPERVITRI™ device can be used in closed or open vitrification mode.

Option 1 – Closed vitrification

• Use the two black marks as a reference for the loading area. Before sealing the SUPERVITRI™, cool the cap for at least 2 seconds by partially immersing it in liquid nitrogen. The opening of the cap must remain above the liquid level.
• Immediately after loading the sample, close the SUPERVITRI™ in the vapor phase above the liquid nitrogen, holding the cap steady.
• Without moving the cap, carefully insert the tip of the device into the pre-cooled cap. Gently push the body forward while simultaneously screwing it in, ensuring a tight seal.
• Verify that, once the device is closed, the arrows printed on the cap and on the body of the SUPERVITRI™ are aligned.
• Immerse the closed device in liquid nitrogen.

Option 2 – Open vitrification

• In the case of open vitrification, use sterile liquid nitrogen and sterilized containers.
• Use the two black marks as a reference for the loading area. Before sealing the SUPERVITRI™, cool the cap for at least 2 seconds by completely immersing it in liquid nitrogen.
• Immediately after loading the sample, immerse first the tip and then the entire SUPERVITRI™ device in liquid nitrogen. Holding the cap steady, carefully insert the tip of the device into the cap while both are immersed in liquid nitrogen. Gently push the body forward while simultaneously screwing it, ensuring a tight seal.
• Verify that, once the device is closed, the arrows printed on the cap and on the body of the SUPERVITRI™ are aligned.

WARMING

• Prepare the warming media according to the recommendations of the selected media kit.
• Remove the SUPERVITRI™ from cryostorage and place it in a transport container, keeping the tip region fully immersed in clean (preferably Sterile Certified) liquid nitrogen.
• Open the SUPERVITRI™ by simultaneously pulling and twisting the cap away from the body.
• Quickly immerse the SUPERVITRI™ tip containing the vitrified oocytes/embryos into the warming solution.
• Under microscopic observation, gently move the SUPERVITRI™ until the oocytes/embryos are released from the tip.
• Complete the warming procedure according to the recommendations of the selected media kit.

This highly effective technique significantly improves the survival rate of oocytes and embryos, increasing the chances of successful fertility treatments.